Coding

Part:BBa_K2486111:Design

Designed by: Fabio Nunes de Mello   Group: iGEM17_USP-Brazil   (2017-10-27)


T-kininogen 1 (no stop codon)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 414
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 468
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence codon optimized for E coli and altered to remove illegal restriction sites.


Source

Originally from the Rattus norvegicus(Norway rat). https://www.ncbi.nlm.nih.gov/gene/288001

References